A simple, precise, accurate, and stability indicating UV-method has been developed and validated for estimation of Metoprolol succinate (METO). UV, HPLC, HPTLC, and many more methods were reported by taking single drug and also by combining with other drugs. However, stress degradation by Uv spectroscopy method was not reported. In both methods, METO has the absorbance maxima at 223 nm. Method A involves method development and validation and Method B involves stress degradation study. In these methods, methanol was used as a solvent. Linearity was observed in the concentration range of 6–24 μg/ml. Validation experiments were performed to demonstrate system suitability, specificity, precision, linearity, accuracy, robustness, LOD, and LOQ as per International Conference on Harmonization guidelines(ICH). Furthermore stability studies of METO were carried out under acidic, alkali, neutral, oxidation, photolytic, and thermal degradation as per stability indicating assay methods. The results of analysis have been validated, and recovery studies were carried out using a standard addition method by adding specific drug amount (50%, 100%, and 150%) and show recovery studies in the range 98.00–100.53%. The proposed method can be successfully applied for method development, validation, and stability study of METO.
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